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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Identification of stabilizing point mutations through mutagenesis of destabilized protein libraries
doi: 10.1016/j.jbc.2022.101785
Figure Lengend Snippet: FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to GyrA14. D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.
Article Snippet: The aggregated protein was removed using centrifugation at 18000 g . The fraction of active protein remaining was measured by binding to
Techniques: Expressing, Binding Assay, Sequencing, Mutagenesis, Fluorescence, FACS